Anti-beta Catenin (phospho S33) antibody (ab73153)

Overview

  • Product nameAnti-beta Catenin (phospho S33) antibody
    See all beta Catenin primary antibodies
  • Description
    Rabbit polyclonal to beta Catenin (phospho S33)
  • Tested applicationsSuitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Catenin, phosphorylated at S33.

    (Peptide available as ab73676.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: U2OS; HeLa; A431; SW480

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab73153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).
IHC-FoFr 1/100.

Target

  • FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similaritiesBelongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Anti-beta Catenin (phospho S33) antibody images

  • All lanes : Anti-beta Catenin (phospho S33) antibody (ab73153) at 1 µg/ml

    Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : SW480 whole cell lysate (ab3957)
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml
    Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml
    Lane 8 : SW480 whole cell lysate (ab3957) with Human beta Catenin (phospho S33) peptide (ab73676) at 1 µg/ml
    Lane 9 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with Human beta Catenin peptide (ab73677) at 1 µg/ml
    Lane 10 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin peptide (ab73677) at 1 µg/ml
    Lane 11 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin peptide (ab73677) at 1 µg/ml
    Lane 12 : SW480 whole cell lysate (ab3957) with Human beta Catenin peptide (ab73677) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 & 12 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Lanes 2 - 11 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution


    Performed under reducing conditions.

    Predicted band size : 85 kDa


    Exposure time : 10 minutes
  • IHC-FoFr image of beta Catenin (phospho S33) staining on rat hippocampal sections (10x and 20x magnification) using ab73153 (1/100). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

References for Anti-beta Catenin (phospho S33) antibody (ab73153)

ab73153 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Unfortunately, because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of any of the antibodies requested. Read More

Thank you for contacting us.

To our knowledge these products have not been tested on canine. However, based on a 99% sequence homology between human each should be viable on canine tissues. I have noted that you have taken advantage of our...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"