Anti-beta Catenin (phospho S33 + S37) antibody [BC-22] (ab11350)


  • Product nameAnti-beta Catenin (phospho S33 + S37) antibody [BC-22]
    See all beta Catenin primary antibodies
  • Description
    Mouse monoclonal [BC-22] to beta Catenin (phospho S33 + S37)
  • SpecificityMonoclonal Anti-phospho-beta-Catenin (pSer33/pSer37) reacts specifically with beta-catenin phosphorylated at Ser37, and Ser33. At the peptide level, the antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphosphorylated peptide. The antibody does not detect the unphosphorylated or the Ser33 phosphorylated protein. It has a stronger affinity for phospho S37 than phospho S33. It does not recognize phosphorylated plakoglobin despite the high homology in the phosphorylation site with beta-catenin.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Synthetic peptide: DSp GIHSpGATTTAPS conjugated to KLH, corresponding to amino acids 32-45 of Human beta Catenin.

  • Positive control
    • Whole extract of cultured 293 (human embryonal kidney) cells treated with the proteasome inhibitor MG132.
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.



Our Abpromise guarantee covers the use of ab11350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 94 kDa. The peptide is highly phosphorylated in the cytosol, and thus is very unstable. When it is translocated to the nucleus, it is at a lower phosphorylation state, and is therefore more stable. You may be seeing degradation products of the protein, since it may be enzymatically degraded in the cytosol. A 50kDa form is present only if the proteosome is inhibited (by MG132 for example)
ICC/IF Use at an assay dependent concentration.
ELISA Use a concentration of 5 - 10 µg/ml.


  • FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similaritiesBelongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

References for Anti-beta Catenin (phospho S33 + S37) antibody [BC-22] (ab11350)

This product has been referenced in:
  • Wang S  et al. Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem cells. J Biol Chem 283:35929-40 (2008). WB ; Mouse, Rabbit . Read more (PubMed: 18940811) »

See 1 Publication for this product

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Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product qu...

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Thank you very much for your call today and for letting us know about the trouble with ab11350.
As we discussed, the lab and I will take a look at your image and I'll forward any comments or suggestions on to you. I'm also sending a free of charge...

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Thank you for contacting us.

To our knowledge these products have not been tested on canine. However, based on a 99% sequence homology between human each should be viable on canine tissues. I have noted that you have taken advantage of our...

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I just received dome feedback from the laboratory who informed me that the antibody is very specific at targeting the phospho S37 form of beta catenin and it binds much better to phospho S37 compared to phospho S33. We expect to see the 50 kDa band...

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Thank you for getting back to me and for providing some useful details of the Western blot experiments. I am very sorry to hear that you are experiencing some difficulties with this antibody. I understand from your e-mail that you used cell lysate ...

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Thank you for your enquiry. I have contacted the originator of this antibody, and they have given me the following information: The peptide is highly phosphorylated in the cytosol, and thus is very unstable. When it is translocated to the nucleu...

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Thank you for contacting us regarding ab11350. I'm afraid I currently do not have any idea as to why you are seeing those bands. It could be non specific bands, or specific bands of the degraded protein, however with limited protocol information I a...

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Thank you for your reply. I had not heard that Tween 20 affected phosphoantibody performance, but I know that certain antibodies call for the omission of Tween from the buffers so it is not out of the question. You should be able to reduce or omit ...

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Thank you for your enquiry. Unfortunately I do not have specific details of a blocking buffer for use by western blot. However, given that this is a phospho antiserum I would recommend the use of BSA as opposed to Milk. I hope this information h...

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