Anti-beta Catenin (phospho Y142) antibody (ab27798)

Overview

  • Product nameAnti-beta Catenin (phospho Y142) antibody
    See all beta Catenin primary antibodies
  • Description
    Rabbit polyclonal to beta Catenin (phospho Y142)
  • SpecificityThe antibody detects beta Catenin on SDS PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate treated cell lines, such as A431 and human endothelial cells. Immunogen sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma Catenin. These human sequences are highly conserved in rat and mouse beta Catenins. Batches could vary in the level phospho Y133 of gamma catenin recognition.
  • Tested applicationsSuitable for: ELISA, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho beta Catenin (Tyr 142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta Catenin.

  • General notes

     

    Tyrosine phosphorylation of beta Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro and overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta Catenins. The phosphorylation of tyrosine 142 may act as a switch from the adhesive to the transcriptional role of beta Catenin. Thus, site specific tyrosine phosphorylation of beta Catenin may regulate specific protein protein interactions, leading to changes in cell adhesion.

Properties

Applications

Our Abpromise guarantee covers the use of ab27798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
WB 1/500. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa).Can be blocked with Human beta Catenin (phospho Y142) peptide (ab42714). IP with beta catenin antibody is recommended, followed by probing with ab27798. This improve the distinction between beta Catenin Tyr 142 and gamma Catenin Tyr 133 (Swiss-Prot ID P14923).
ICC 1/50.

Target

  • FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similaritiesBelongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Anti-beta Catenin (phospho Y142) antibody images

  • ICC/IF labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin (left panel) and ab27798 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

  • ab27798 staining beta Catenin (phospho Y142) in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence. The cells were pre-treated with pervanadate for 15 minutes before being fixed in paraformaldehyde and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 8 hours at 4°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) ab150076) used at a 1/500 dilution. Hoestch was used to stain the cell nuclei (blue).

  • Lanes 1 - 2 : anti beta catenin
    Lanes 3 - 4 : Anti-beta Catenin (phospho Y142) antibody (ab27798) at 1/500 dilution

    Lane 1 : Hct116 src transformed cells serum starved overnight.
    Lane 2 : Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min.
    Lane 3 : Hct116 src transformed cells serum starved overnight.
    Lane 4 : Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min.

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 86 kDa
    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

References for Anti-beta Catenin (phospho Y142) antibody (ab27798)

This product has been referenced in:
  • DiStefano PV  et al. KRIT1 protein depletion modifies endothelial cell behavior via increased vascular endothelial growth factor (VEGF) signaling. J Biol Chem 289:33054-65 (2014). WB . Read more (PubMed: 25320085) »
  • Mishra J  et al. Role of Janus kinase 3 in mucosal differentiation and predisposition to colitis. J Biol Chem 288:31795-806 (2013). WB ; Mouse . Read more (PubMed: 24045942) »

See all 10 Publications for this product

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Thank you for contacting us. The pervanadate treatment protocol is as follows:

Reagents:

Pervanadate (100 mM stock)
100 µl 30% Hydrogen Peroxide(H2O2)
200 µl 500 mM Sodium Vanadate
700 µl dH2O
<...

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Application Western blot
Sample Mouse Cell lysate - other (immunoprecipitated b-catenin from mES cells)
Loading amount 1e+006 cells
Specification immunoprecipitated b-catenin from mES cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Dr. Penny Hayward

Verified customer

Submitted Jan 17 2012

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I have obtained an explanation for you. I hope it is satisfactory! ab27798 immunogen sequence (amino acid residues around tyrosine 142 of human beta ) has one amino acid difference from a sequence around tyrosine 133 of human gamma Catenin. Thus, ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (Hippocampal neurons)
Specification Hippocampal neurons
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Judit Herreros

Verified customer

Submitted Apr 01 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela S3 cells)
Loading amount 30 µg
Specification Hela S3 cells
Treatment +/- [30 mM] Pervanadate for 30 min
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

Submitted Nov 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (pancreas)
Loading amount 30 µg
Specification pancreas
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Alessandra Strom

Verified customer

Submitted May 10 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"