Anti-beta Crystallin antibody [3.H9.2] (ab90379)

Overview

  • Product nameAnti-beta Crystallin antibody [3.H9.2]
  • Description
    Mouse monoclonal [3.H9.2] to beta Crystallin
  • Specificityab90379 detects beta Crystallin. It may show weak reactivity with alpha Crystallin A.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rabbit, Cow, Xenopus laevis
  • Immunogen

    Full length native bovine beta Crystallin.

  • Positive control
    • Bovine beta Crystallin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, 0.1mM PMSF, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody was purified using Protein G affinity chromatography.
  • ClonalityMonoclonal
  • Clone number3.H9.2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.

Target

  • FunctionCrystallins are the dominant structural components of the vertebrate eye lens.
  • Involvement in diseaseDefects in CRYBB2 are the cause of cataract congenital cerulean type 2 (CCA2) [MIM:601547]; also known as congenital cataract blue dot type 2. A cerulean form of autosomal dominant congenital cataract. Cerulean cataract is characterized by peripheral bluish and white opacifications organized in concentric layers with occasional central lesions arranged radially. The opacities are observed in the superficial layers of the fetal nucleus as well as the adult nucleus of the lens. Involvement is usually bilateral. Visual acuity is only mildly reduced in childhood. In adulthood, the opacifications may progress, making lens extraction necessary. Histologically the lesions are described as fusiform cavities between lens fibers which contain a deeply staining granular material. Although the lesions may take on various colors, a dull blue is the most common appearance and is responsible for the designation cerulean cataract.
    Defects in CRYBB2 are the cause of cataract sutural with punctate and cerulean opacities (CSPC) [MIM:607133]. A form of cataract characterized by white opacification around the anterior and posterior Y sutures, and grayish and bluish, spindle shaped, oval punctate and cerulean opacities of various sizes arranged in lamellar form. The spots are more concentrated towards the peripheral layers and do not delineate the embryonal or fetal nucleus. Phenotypic variation with respect to the size and density of the sutural opacities as well as the number and position of punctate and cerulean spots is observed among affected subjects.
    Defects in CRYBB2 are a cause of cataract Coppock-like (CCL) [MIM:604307]. A congenital pulverulent disk-like opacity involving the embryonic nucleus with many tiny white dots in the lamellar portion of the lens. It is usually bilateral and dominantly inherited.
  • Sequence similaritiesBelongs to the beta/gamma-crystallin family.
    Contains 4 beta/gamma crystallin 'Greek key' domains.
  • DomainHas a two-domain beta-structure, folded into four very similar Greek key motifs.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta crystallin B2 antibody
    • Beta crystallin Bp antibody
    • Beta-B2 crystallin antibody
    • Beta-crystallin B2 antibody
    • Beta-crystallin Bp antibody
    • CCA 2 antibody
    • CCA2 antibody
    • CRBB2_HUMAN antibody
    • CRY B2 antibody
    • CRYB 2 antibody
    • CRYB B2 antibody
    • CRYB2 antibody
    • CRYB2A antibody
    • CRYBB 2 antibody
    • CRYBB2 antibody
    • Crystallin beta B2 antibody
    • D22S665 antibody
    • Eye lens structural protein antibody
    • OTTHUMP00000198622 antibody
    see all

References for Anti-beta Crystallin antibody [3.H9.2] (ab90379)

This product has been referenced in:
  • Pai VP  et al. Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis. Development 139:313-23 (2012). ICC/IF ; Xenopus laevis . Read more (PubMed: 22159581) »

See 1 Publication for this product

Product Wall

We have not specifically tested against various forms of Beta crystallin so I cannot specifically comment.

We do have one antibody specific for CRYBB1, catalog number ab54655 http://www.abcam.com/beta-b1-crystallin-antibody-ab54655.html

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"