The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa. Used in conjunction with compatible secondary reagents the detection limit for ab109571 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). ab109572, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta Defensin 3.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to beta Defensin 3 (Biotin) (ab84234). (using 100 µl/well antibody solution). ab109572, in conjunction with ab84234 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta Defensin 3.
FunctionExhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
Tissue specificityHighly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
Sequence similaritiesBelongs to the beta-defensin family.