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Our Abpromise guarantee covers the use of ab19270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23627872|
ab19270 staining beta Defensin 3 in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An Alexa Fluor®488-conjugated was used as the secondary antibody and DAPI used to stain the nuclei.