Anti-beta Dystroglycan antibody (ab43125)

Overview

  • Product name
    Anti-beta Dystroglycan antibody
    See all beta Dystroglycan primary antibodies
  • Description
    Rabbit polyclonal to beta Dystroglycan
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Dystroglycan precursor.

    (Peptide available as ab43750.)

  • Positive control
    • ab43125 gave a positive signal in the following human tissue lysates: Brain; Heart; Skeletal Muscle; Testes (data not shown); Kidney (data not shown); Colon (data not shown)

Properties

Applications

Our Abpromise guarantee covers the use of ab43125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Relevance
    Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
  • Cellular localization
    Type I membrane protein.
  • Database links
  • Alternative names
    • 156DAG antibody
    • A3a antibody
    • AGRNR antibody
    • Beta DG antibody
    • Beta dystroglycan antibody
    • DAG 1 antibody
    • DAG1 antibody
    • Dystroglycan antibody
    • Dystrophin associated glycoprotein 1 antibody
    see all

Anti-beta Dystroglycan antibody images

  • All lanes : Anti-beta Dystroglycan antibody (ab43125) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References for Anti-beta Dystroglycan antibody (ab43125)

ab43125 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skeletal muscle (tibialis anterior muscle))
Permeabilization
Yes - 0.1 % Triton X-100
Specification
skeletal muscle (tibialis anterior muscle)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 23 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
no fixative
Username

Miss. Laurence Richard

Verified customer

Submitted Sep 23 2015

Have you tried increasing the concentration of ab64568? It does look like the MAb is reacting at the correct area but there does appear to be some issue which is affecting the antibody/antigen binding and preventing good visualization. Have you attempt...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Oct 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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