Anti-beta Dystroglycan antibody [EP2200Y] (ab62373)

Overview

  • Product nameAnti-beta Dystroglycan antibody [EP2200Y]
    See all beta Dystroglycan primary antibodies
  • Description
    Rabbit monoclonal [EP2200Y] to beta Dystroglycan
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human beta Dystroglycan

  • Positive control
    • Human brain lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab62373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 - 1/2000. Detects a band of approximately 43 kDa.
    Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).

    Is unsuitable for Flow Cyt, ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
    • Cellular localizationType I membrane protein.
    • Database links
    • Alternative names
      • 156DAG antibody
      • A3a antibody
      • AGRNR antibody
      • Beta DG antibody
      • Beta dystroglycan antibody
      • DAG 1 antibody
      • DAG1 antibody
      • Dystroglycan antibody
      • Dystrophin associated glycoprotein 1 antibody
      see all

    Anti-beta Dystroglycan antibody [EP2200Y] images

    • Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/2000 dilution + human brain lysate at 10 µg

      Secondary
      Goat anti-rabbit, HRP labeled, at 1/2000 dilution

    References for Anti-beta Dystroglycan antibody [EP2200Y] (ab62373)

    ab62373 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab62373.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"