Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

Overview

  • Product name
    Anti-beta Dystroglycan (phospho Y892) antibody [M117]
    See all beta Dystroglycan primary antibodies
  • Description
    Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)
  • Specificity
    Ab29037 recognises beta Dystroglycan (phospho Y892).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 892 of human dystroglycan.

  • Positive control
    • HepG2 cells or HeLa cells treated with pervanadate.

Properties

Applications

Our Abpromise guarantee covers the use of ab29037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
    • Cellular localization
      Type I membrane protein.
    • Database links
    • Alternative names
      • 156DAG antibody
      • A3a antibody
      • AGRNR antibody
      • Beta DG antibody
      • Beta dystroglycan antibody
      • DAG 1 antibody
      • DAG1 antibody
      • Dystroglycan antibody
      • Dystrophin associated glycoprotein 1 antibody
      see all

    Images

    • All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

      Lane 1 : Lysates from HepG2 cells (untreated)
      Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 97 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    • Predicted band size : 97 kDa

    References

    ab29037 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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