Anti-beta Galactosidase antibody [2E9] (ab10074)

Overview

  • Product nameAnti-beta Galactosidase antibody [2E9]
    See all beta Galactosidase primary antibodies
  • Description
    Mouse monoclonal [2E9] to beta Galactosidase
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length protein (expressed in E. coli).

  • General notesThis antibody does not influence enzyme activity. Concentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone number2E9
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesIA: Detection of b-galactosidase expressed by LacZ gene in cloned sequences.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • Cellular localizationCytoplasmic
    • Database links
      • Alternative names
        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      References for Anti-beta Galactosidase antibody [2E9] (ab10074)

      ab10074 has not yet been referenced specifically in any publications.

      Product Wall

      Thank you for your reply. I have checked the sequence homology between beta-galactosidase of E.coli (P00722) with Aspergillus oryzae (CAW30743.1). The alignment is very poor and shows only 22-33% homology for short regions, otherwise the homolo...

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      Thank you for contacting us. I am checking with the lab regarding ab92417 and if cross reactivity with alpha-galactosidase has been tested, and will let you know as soon as I hear back. For both antibodies (ab92417, ab10074): Both were o...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"