Anti-beta Galactosidase antibody (ab107114)

Overview

  • Product name
    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Chicken polyclonal to beta Galactosidase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Escherichia coli beta Galactosidase

  • Positive control
    • This antibody gave a positive signal when tested against B-galactosidase from E. coli.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 3% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).

Target

  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Images

    • Anti-beta Galactosidase antibody (ab107114) at 1 µg/ml + B-galactosidase from E. coli at 10 µg

      Secondary
      Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 116 kDa
      Observed band size : 116 kDa
      Additional bands at : >250 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

    References

    ab107114 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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