Overview

  • Product name
    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Goat polyclonal to beta Galactosidase
  • Specificity
    Detects recombinant beta galactosidase enzyme by Western blotting.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein of Escherichia coli beta Galactosidase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.025% Sodium azide
    Constituent: PBS

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.

Target

  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Anti-beta Galactosidase antibody images

    • Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 116 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 90 seconds

    References for Anti-beta Galactosidase antibody (ab12081)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (bact beta-gal protein expressed in rat epithelial)
    Loading amount
    20 µg
    Specification
    bact beta-gal protein expressed in rat epithelial
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Dr. Silvia Finnemann

    Verified customer

    Submitted Aug 13 2012

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not with the full amount as stated on the datasheet and for the inconvenience...

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    Thank you for your phone call.

    As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with...

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    Thank you for your email. Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane. Looking forward to your re...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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