Overview

  • Product nameAnti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Rabbit polyclonal to beta Galactosidase
  • Tested applicationsSuitable for: ELISA, WB, IPmore details
    Unsuitable for: ICC/IF,IHC-FoFr,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein (E. coli).

  • Positive control
    • 1.0µg of E.coli in a standard sandwich ELISA. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified E.coli beta-galactosidase.
  • General notes

    Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesIgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation and ion exchange chromatography and dialysis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.

sELISA tested against 1ug E.coli beta galactosidase.

WB 1/5000 - 1/10000. Detects a band of approximately 116 kDa.

This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives.

IP 1/500 - 1/5000.
  • Application notesIs unsuitable for ICC/IF,IHC-FoFr,IHC-Fr or IHC-P.
  • Target

    • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • Cellular localizationCytoplasmic
    • Database links
      • Alternative names
        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      Anti-beta Galactosidase antibody images

      • Immunostaining of beta galactosidase (brown) in paraffin embedded, 4% PFA fixed, mouse brain (SVZ, AL8 mice) using ab616. Olig2 is stained in blue. Heat medidated antigen retrieval in Tris buffer (pH 8.0) was carried out and then beta galactosidase was visualised using a HRP based DAB staining kit.

      • Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.

      References for Anti-beta Galactosidase antibody (ab616)

      This product has been referenced in:
      • Sahu-Osen A  et al. CGI-58/ABHD5 is phosphorylated on Ser239 by protein kinase A: control of subcellular localization. J Lipid Res 56:109-21 (2015). WB . Read more (PubMed: 25421061) »
      • Szabó NE  et al. Cadherins mediate sequential roles through a hierarchy of mechanisms in the developing mammillary body. Front Neuroanat 9:29 (2015). IHC-P . Read more (PubMed: 25852491) »

      See all 68 Publications for this product

      Product Wall

      I am very pleased to hear you would like to accept our offer and testab616 and ab15580in ICE. These codes will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for ICE for each antib...

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      Western blot

      Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Western blot
      Loading amount 10 µg
      Gel Running Conditions Reduced Denaturing
      Sample Mouse Tissue lysate - whole (epidermis)
      Specification epidermis
      Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Username

      Abcam user community

      Verified customer

      Submitted May 29 2013

      Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
      Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
      Sample Mouse Tissue sections (epidermis)
      Specification epidermis
      Permeabilization No
      Fixative Paraformaldehyde
      Username

      Abcam user community

      Verified customer

      Submitted May 28 2013

      Application Immunocytochemistry/ Immunofluorescence
      Sample Mouse Cell (ROSA LacZ/+ AMH-Cre adult testes)
      Specification ROSA LacZ/+ AMH-Cre adult testes
      Fixative 4 ? Acetone:Methanol 1:1
      Permeabilization No
      Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Username

      Dr. Qing Wen

      Verified customer

      Submitted Dec 20 2012

      Merci de nous avoir contactés.

      J'ai pu voir d'après nos archives que plusieurs utilisateurs ont rencontré des problèmes avec cet anticorps en IHC-P. L'application IHC-P a d'ailleurs été retirée ...

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      Thank you for submitting an Abreview for ab616. As you may have noticed, your review has now been published on our website.
      Since you obtained poor results using the antibody in an untested species, we would like to follow up on this to see if we c...

      Read More
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application ELISA
      Sample Human Cell (H407 cells)
      Specification H407 cells
      Blocking step AbCam ICE kit as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
      Type Indirect
      Username

      Abcam user community

      Verified customer

      Submitted Oct 10 2012

      Thank you for contacting us yesterday.

      I am sorry to hear you are experiencing difficulties with one of our products. As discussed over the phone, I'd like to try and help you to improve the results currently obtained. In order to do this, c...

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      Vielen Dank für Ihre Anfrage.

      Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so...

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      Merci pour ces précisions.

      Je vous souhaite une bonne continuation dans vos expérimentations. N'hésitez pas à nous contacter de nouveau si vous avez des questions.

      1-10 of 74 Abreviews or Q&A

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"