Overview

  • Product name
    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Rabbit polyclonal to beta Galactosidase
  • Tested applications
    Suitable for: ELISA, WB, IPmore details
    Unsuitable for: ICC/IF,IHC-FoFr,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein (E. coli).

  • Positive control
    • 1.0µg of E.coli in a standard sandwich ELISA. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified E.coli beta-galactosidase.
  • General notes

    Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.

sELISA tested against 1ug E.coli beta galactosidase.

WB 1/5000 - 1/10000. Detects a band of approximately 116 kDa.

This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives.

IP 1/500 - 1/5000.
  • Application notes
    Is unsuitable for ICC/IF,IHC-FoFr,IHC-Fr or IHC-P.
  • Target

    • Relevance
      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • Cellular localization
      Cytoplasmic
    • Database links
      • Alternative names
        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      Images

      • Immunostaining of beta galactosidase (brown) in paraffin embedded, 4% PFA fixed, mouse brain (SVZ, AL8 mice) using ab616. Olig2 is stained in blue. Heat medidated antigen retrieval in Tris buffer (pH 8.0) was carried out and then beta galactosidase was visualised using a HRP based DAB staining kit.

      • Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.

      References

      This product has been referenced in:
      • Lee JJ  et al. Control of inflammation by stromal Hedgehog pathway activation restrains colitis. Proc Natl Acad Sci U S A 113:E7545-E7553 (2016). Read more (PubMed: 27815529) »
      • Bastakoty D  et al. Temporary, Systemic Inhibition of the WNT/ß-Catenin Pathway promotes Regenerative Cardiac Repair following Myocardial Infarct. Cell Stem Cells Regen Med 2:N/A (2016). Read more (PubMed: 28042617) »

      See all 92 Publications for this product

      Customer reviews and Q&As

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      Western blot

      Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (epidermis)
      Specification
      epidermis
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Username

      Abcam user community

      Verified customer

      Submitted May 29 2013

      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate
      Sample
      Mouse Tissue sections (epidermis)
      Specification
      epidermis
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Username

      Abcam user community

      Verified customer

      Submitted May 28 2013

      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (ROSA LacZ/+ AMH-Cre adult testes)
      Specification
      ROSA LacZ/+ AMH-Cre adult testes
      Fixative
      4 ? Acetone:Methanol 1:1
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Username

      Dr. Qing Wen

      Verified customer

      Submitted Dec 20 2012

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      ELISA
      Sample
      Human Cell (H407 cells)
      Specification
      H407 cells
      Blocking step
      AbCam ICE kit as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
      Type
      Indirect
      Username

      Abcam user community

      Verified customer

      Submitted Oct 10 2012

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Heart)
      Loading amount
      40 µg
      Specification
      Heart
      Gel Running Conditions
      Reduced Denaturing (8 % gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Username

      Abcam user community

      Verified customer

      Submitted Feb 01 2012

      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Fruit fly (Drosophila melanogaster) Cell (wing)
      Specification
      wing
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1 % Triton X 100
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Username

      Abcam user community

      Verified customer

      Submitted Jul 14 2011

      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (carotid artery)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate
      Permeabilization
      Yes - PBS w 1% Saponin
      Specification
      carotid artery
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Fixative
      Formaldehyde
      Username

      Abcam user community

      Verified customer

      Submitted Apr 25 2011

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Escherichia coli Cell lysate - whole cell (HEK293)
      Loading amount
      15 µg
      Specification
      HEK293
      Treatment
      transfected with lacZ-overexpressing plasmid
      Gel Running Conditions
      Reduced Denaturing ('any Kd' Biorad precast)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Username

      Abcam user community

      Verified customer

      Submitted Nov 01 2010

      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      None
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Username

      Abcam user community

      Verified customer

      Submitted Sep 14 2010

      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Skin)
      Specification
      Skin
      Fixative
      Methanol
      Permeabilization
      No
      Blocking step
      Horse Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Username

      Abcam user community

      Verified customer

      Submitted Jul 16 2010

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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