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Immunohistochemistry (PFA perfusion fixed frozen sections) - beta Galactosidase antibody (ab9361)
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Immunohistochemistry (Frozen sections) - beta Galactosidase antibody (ab9361)
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Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab9361)
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Product Name
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beta Galactosidase antibody
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See all beta Galactosidase antibodies (15)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to beta Galactosidase
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Immunogen
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Full length native protein (purified) (E. coli).
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Reacts with
(species key)
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E coli
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, Ie
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Ie: Use at an assay dependent dilution.
IHC-P: 1/200 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: 1/1000 - 1/2000.
IHC-FrFl: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase
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Relevance
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Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 945006 (Escherichia coli )
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| SwissProt | | P00722 (Escherichia coli )
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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0.01M PBS pH7.4, 15mM Na azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Galactosidase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for beta Galactosidase antibody images, references, products related to ab9361 and other tools.
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beta Galactosidase antibody images:
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 Immunohistochemistry (PFA perfusion fixed frozen sections) - beta Galactosidase antibody (ab9361)
ab9361 at 1/500 dilution staining mouse adult brain cells (R26R mice crossed with cre expressing line) by Immunocytochemistry. The cells were 4% PFA perfused 4H post-fix. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using a Alexa Fluor ® 488 conjugated goat-anti chicken antibody. The image shows both nuclear and punctate staining (both matched x-gal staining pattern). This image is courtesy of an Abreview submitted by Ben Deverman.
See Abreview
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Immunohistochemistry (Frozen sections) - beta Galactosidase antibody (ab9361)
ab9361 at 1/1000 staining frozen mouse pancreas sections by IHC-Fr. The mice were transgenic knock out or heterozygous (in this case LRH +/-)with Lacz reporter replacing a section of the coding sequence for gene. Embryos collected at day E14.5. The tissue was formaldehyde fixed and blocked with a maleate buffer blocking solution prior to incubation with the antibody for 16 hours. A Cy3 ® conjugated goat anti-chicken antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr Asha Seth
See Abreview
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Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab9361)
ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr Deon Wolpowitz
See Abreview
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References for beta Galactosidase antibody (ab9361)
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This product has been used in: (two most recent references)
Storm R et al. The bHLH transcription factor Olig3 marks the dorsal neuroepithelium of the hindbrain and is essential for the development of brainstem nuclei. Development 136:295-305 (2009). PubMed: 19088088
Papaioannou MD et al. Sertoli cell Dicer is essential for spermatogenesis in mice. Dev Biol 326:250-9 (2009). IHC-P. PubMed: 19071104
See all 27 publication references for this product.
If you publish research using ab9361 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to beta Galactosidase
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beta Galactosidase antibody - more information
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