Anti-beta Galactosidase antibody [BGAL01] (ab3291)

Overview

  • Product nameAnti-beta Galactosidase antibody [BGAL01]
    See all beta Galactosidase primary antibodies
  • Description
    Mouse monoclonal [BGAL01] to beta Galactosidase
  • Tested applicationsSuitable for: IP, WBmore details
  • Immunogen

    Full length protein (E. coli).

  • Positive control
    • Purified recombinant / beta Galactosidase protein or transfected cell lines expressing beta Galactosidase tagged proteins.
  • General notesMolecular weight of beta Galactosidase = ~ 116 kDa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberBGAL01
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesImmunoprecipitation (with protein G): use at a concentration of 2µg / mg protein lysate.
    Western Blotting: use at a concentration of 1µg / ml for 2hrs at RT.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    This antibody may be used for detection of beta Galactosidase expressed by the E.coli lacZ geneencoded in many cloned gene sequences, and servesas an indicator for fusion proteins encoded by aninserted DNA sequence.
  • Target

    • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • Cellular localizationCytoplasmic
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    References for Anti-beta Galactosidase antibody [BGAL01] (ab3291)

    ab3291 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"