Anti-beta Galactosidase antibody [DC1 4C7] (ab116)

Overview

  • Product name
    Anti-beta Galactosidase antibody [DC1 4C7]
    See all beta Galactosidase primary antibodies
  • Description
    Mouse monoclonal [DC1 4C7] to beta Galactosidase
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    beta Galactosidase EC.3.2.1.23 from E.coli.

  • General notes

    This monoclonal is also known as clone HD4C7 or HD 447C.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    DC1 4C7
  • Myeloma
    P3-NS1/1-Ag4-1
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

Images

  • All lanes : Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg

    Lane 1 : Recombinant Human GLB1 protein (ab151890)
    Lane 2 : Recombinant E. coli beta Galactosidase protein (ab79449)

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time : 4 minutes

References

This product has been referenced in:
  • Smith SJ  et al. Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition. PLoS One 7:e52335 (2012). IHC-P . Read more (PubMed: 23272238) »
  • Nassiri M  et al. Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice. Arterioscler Thromb Vasc Biol 30:1423-9 (2010). IHC-Fr . Read more (PubMed: 20431063) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We do not have a recommended dilution of this antibody. The dilution employed will depend on the level of expression of your LacZ construct. H...

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Thank you for your enquiry and for submitting your technical questionnaire. I am sorry to hear that your customer has been having difficulties with this antibody. I have read their technical enquiry and they have tried some of the recommendations th...

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Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We would like to confirm that this antibody has not been tested for immunohistochemi...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Yes, ab616 has successfully been tested for application in IHC-formalin fixed paraffin embedded sections. We have had some feedback from customers who have used ab616 in IHC-P to detect beta gal in transgenic mice - you may read their comments by click...

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Thank you for your email. Neither ab116 or ab1047 have been tested yet for application in IHC. You may want to look at ab616 which has been tested in IHC. If you decide to try ab116 or ab1047 in IHC, please let us know how you get on and in return w...

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This anitbody has not yet been tested in immunocytochemistry

Yes this antibody does work in this application (although there maybe some post-translational modification of the b-gal fusion protein which may decrease the binding of the antibody)

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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