Anti-beta Galactosidase antibody [DC1 4C7] (ab116)
Key features and details
- Mouse monoclonal [DC1 4C7] to beta Galactosidase
- Suitable for: WB, ELISA
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-beta Galactosidase antibody [DC1 4C7]
See all beta Galactosidase primary antibodies -
Description
Mouse monoclonal [DC1 4C7] to beta Galactosidase -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
This monoclonal is also known as clone HD4C7 or HD 447C.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DC1 4C7 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Images
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All lanes : Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg
Lane 1 :Recombinant Human GLB1/Beta-galactosidase protein (ab151890)
Lane 2 :Native E. coli beta Galactosidase protein (ab79449)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 4 minutes
Datasheets and documents
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Datasheet download
References (7)
ab116 has been referenced in 7 publications.
- Tripathi BK et al. Inhibition of cytoplasmic EZH2 induces antitumor activity through stabilization of the DLC1 tumor suppressor protein. Nat Commun 12:6941 (2021). PubMed: 34862367
- Inaba M et al. Nanotubes mediate niche-stem-cell signalling in the Drosophila testis. Nature 523:329-32 (2015). PubMed: 26131929
- Smith SJ et al. Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition. PLoS One 7:e52335 (2012). IHC-P . PubMed: 23272238
- Nassiri M et al. Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice. Arterioscler Thromb Vasc Biol 30:1423-9 (2010). IHC-Fr . PubMed: 20431063
- Büchmann-Møller S et al. Multiple lineage-specific roles of Smad4 during neural crest development. Dev Biol 330:329-38 (2009). PubMed: 19361496
- Kleinschmidt AM et al. Sequences in intron 51 of the von Willebrand factor gene target promoter activation to a subset of lung endothelial cells in transgenic mice. J Biol Chem 283:2741-50 (2008). IHC-Fr . PubMed: 18048367
- Li S et al. Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting. Genesis 41:73-80 (2005). IHC . PubMed: 15682388