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The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ.
Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase.
Visit our FAQs page for tips and troubleshooting.
This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (ab65351).
|Fixative Solution (1X)||NM||1 x 125ml|
|Staining Solution (1X)||WM||1 x 125ml|
|Staining Supplement (100X)||Red||1 x 1.5ml|
|X-Gal||Green||1 x 150mg|
Our Abpromise guarantee covers the use of ab102534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Image obtained from Li XH et al; PLOS One; 2012;7(5):e36604