Abcam's beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390) utilizes beta HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 µL samples or 0.01-0.2 mM of beta HB samples.
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Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
|beta HB Assay Buffer||WM||1 x 25ml|
|beta HB Enzyme Mix (lyophilised)||Green||1 vial|
|beta HB Standard (1.0 µmol) (lyophilised)||Yellow||1 vial|
|beta HB Substrate mix (lyophilised)||Red||1 vial|
Our Abpromise guarantee covers the use of ab83390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Beta hydroxybutyrate in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Beta hydroxybutyrate measured in biological fluids showing quantity (nmol) per microliter of tested sample
Human serum samples were filtered using a 10 kda MW cut-off spin filter (ab93349) spun at 14,000 RPM for 30 minutes. Beta Hydrobutyrate (BHB) was detected on a 96-well plate using ab83390.
Young control group (6 men, 25±1 years old).
Age-matched control group (13 men, 69±7 years old)
PD = Individuals diagnosed with idiopathic PD (15 men, 71±8 years old, duration of disease = 10±6 years, H&Y = 2.3±0.7, median = 2).
A trend approaching significance in increased levels of BHB in the plasma of PD patients compared to age-matched controls was observed. BHB is known to fluctuate in a variety of conditions including starvation, diabetes mellitus and other co-morbidities.
The plasma levels of total BHB was detected using ab83390. Five rats were in each group. Samples were collected from tail vein blood and were transferred into EDTA-containing tubes. After centrifugation at 1500×g for 15 min at room temperature, the plasma was transferred into a new tube and stored at -80°C until use. Values are presented as the mean ± S.E. (**p<0.01).