• Product nameAnti-beta I Tubulin antibody
    See all beta I Tubulin primary antibodies
  • Description
    Mouse polyclonal to beta I Tubulin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human beta I Tubulin protein (NP_110400.1)

  • Positive control
    • Rat brain lysate; beta I Tubulin transfected 293T cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab88257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.


  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Involvement in diseaseDefects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810484G07Rik antibody
    • Beta tubulin 1, class VI antibody
    • Class VI beta tubulin antibody
    • dJ543J19.4 antibody
    • M(beta)1 antibody
    • TBB1_HUMAN antibody
    • TUBB1 antibody
    • Tubulin beta 1 class VI antibody
    • Tubulin beta-1 chain antibody
    • Tubulin, beta 1 antibody
    • tubulin, beta1 antibody
    see all

Anti-beta I Tubulin antibody images

  • All lanes : Anti-beta I Tubulin antibody (ab88257) at 1 µg/ml

    Lane 1 : beta I Tubulin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Anti-beta I Tubulin antibody (ab88257) at 1 µg/ml + Rat brain lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)

References for Anti-beta I Tubulin antibody (ab88257)

ab88257 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88257.
Please use the links above to contact us or submit feedback about this product.