Overview

  • Product nameAnti-beta I Tubulin antibody
    See all beta I Tubulin primary antibodies
  • Description
    Rabbit polyclonal to beta I Tubulin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 174 - 438 of beta I Tubulin.

  • Positive control
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab96008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Involvement in diseaseDefects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810484G07Rik antibody
    • Beta tubulin 1, class VI antibody
    • Class VI beta tubulin antibody
    • dJ543J19.4 antibody
    • M(beta)1 antibody
    • TBB1_HUMAN antibody
    • TUBB1 antibody
    • Tubulin beta 1 class VI antibody
    • Tubulin beta-1 chain antibody
    • Tubulin, beta 1 antibody
    • tubulin, beta1 antibody
    see all

Anti-beta I Tubulin antibody images

  • All lanes : Anti-beta I Tubulin antibody (ab96008) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96008 at 1/200 dilution.
    Bottom panel: merged with DNA probe.

References for Anti-beta I Tubulin antibody (ab96008)

This product has been referenced in:
  • Wang H  et al. Multiple mechanisms underlying acquired resistance to taxanes in selected docetaxel-resistant MCF-7 breast cancer cells. BMC Cancer 14:37 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24447372) »

See 1 Publication for this product

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It is clear that tag is a problem which is interfering with the antibody. It may be that due to stearic hindrance epitope is not fully exposed because of conformational changes in the target protein.

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I am sorry we have not tested any of anti tubilin I antibodies in GFP-tubulin 1 tag so we will not be able to recommend any specific antibody. Surely, the heavy tag like GFP affects the binding of antibody to prote...

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I will forward these details on to our Distributor’s Team who would be happy to organise the free of charge replacement. Your customer may be interested to know that if needs be, double staining can be performed with primary antibodies from the sam...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"