Recombinant
RabMAb

Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Overview

  • Product name
    Anti-beta I Tubulin antibody [EPR16778]
    See all beta I Tubulin primary antibodies
  • Description
    Rabbit monoclonal [EPR16778] to beta I Tubulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta I Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H4B7

  • Positive control
    • WB: Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, Rat brain and Rat spleen lysates; K562, Jurkat, HeLa, 293T, C6, RAW 264.7, PC-12, NIH/3T3, UMNSAH/DF-1, BHK, MDCK, MDBK, and COS-1 whole cell lysates. IHC-P: Human tonsil tissue; Mouse cerebral cortex tissue; Rat spleen tissue. ICC/IF: K562 cells. FC: K562 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16778
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Involvement in disease
    Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810484G07Rik antibody
    • Beta tubulin 1, class VI antibody
    • Class VI beta tubulin antibody
    • dJ543J19.4 antibody
    • M(beta)1 antibody
    • TBB1_HUMAN antibody
    • TUBB1 antibody
    • Tubulin beta 1 class VI antibody
    • Tubulin beta-1 chain antibody
    • Tubulin, beta 1 antibody
    • tubulin, beta1 antibody
    see all

Images

  • All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/20000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 4 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/1000 dilution

    Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates
    Lane 3 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 4 : MDBK (Bovine kidney cell line) whole cell lysates
    Lane 5 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab179511 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin. 

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab179511 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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