Overview

  • Product nameAnti-beta I Tubulin antibody [SAP.4G5]See all beta I Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [SAP.4G5] to beta I Tubulin
  • SpecificityNo reactivity with other tubulin isotypes is observed. The antibody is specific for beta I and does not recognize beta II, III, or IV by western blot. Beta VI does contain the sequence. However, there is no experimental proof that the antibody recognizes this isoform.
  • Tested applicationsIHC-P, ELISA, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to the C-terminal sequence of beta-tubulin isotype I, coupled to BSA.

  • EpitopeAb11312 specifically recognizes an epitope located in the C-terminal sequence of beta tubulin isotype I of human, bovine, dog, hamster, rat, mouse, chicken and Xenopus.
  • Positive control
    • Cultured chicken fibroblasts.
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • PurityAscites
  • Clonality Monoclonal
  • Clone numberSAP.4G5
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab11312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
ELISA
ICC/IF
WB
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (methanolacetone and 3% paraformaldehyde-0.5% Triton-x-100 fixations).
    IHC-P: Use at an assay dependent dilution (10% neutral buffered formalin-alcoholic formalin-xylene, paraffin-embedded).
    WB: 1/20000, this dilution is determined using cultured chicken fibroblasts. Predicted molecular weight: 55kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
    • Involvement in diseaseDefects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
    • Sequence similaritiesBelongs to the tubulin family.
    • Post-translational
      modifications
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Target information above from: UniProt accession Q9H4B7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 2810484G07Rik antibody
      • Beta tubulin 1, class VI antibody
      • Class VI beta tubulin antibody
      • dJ543J19.4 antibody
      • M(beta)1 antibody
      • TBB1_HUMAN antibody
      • TUBB1 antibody
      • Tubulin beta 1 class VI antibody
      • Tubulin beta-1 chain antibody
      • Tubulin, beta 1 antibody
      • tubulin, beta1 antibody
      see all

    Anti-beta I Tubulin antibody [SAP.4G5] images

    • ab11312 (1µg/ml) staining beta 1 tubulin in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    References for Anti-beta I Tubulin antibody [SAP.4G5] (ab11312)

    This product has been referenced in:
    • McCarroll JA  et al. betaIII-tubulin is a multifunctional protein involved in drug sensitivity and tumorigenesis in non-small cell lung cancer. Cancer Res 70:4995-5003 (2010). WB ; Human . Read more (PubMed: 20501838) »
    • Davis B  et al. Mutation in beta1-tubulin correlates with macrothrombocytopenia in Cavalier King Charles Spaniels. J Vet Intern Med 22:540-5 (2008). Read more (PubMed: 18466252) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your enquiry.

    I can confirm that Anti-beta I Tubulin antibody [SAP.4G5] (ab11312) is sold as ascites. Unpurified antibodies, such as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on t...

    Read More

    Thank you for your enquiry. The sequence is cited in the article shows that the antibody is specific for beta I, and does not recognize beta II, III or IV, by western blot. Indeed, beta V contains the seq., however, there is no experimental proof...

    Read More

    Thank you for your enquiry. This antibody clone has been applied in by western blotting in the following reference; Wang Y et al. Protease-activated receptor-1 protects rat astrocytes from apoptotic cell death via JNK-mediated release of the ch...

    Read More

    Thank you for your enquiry. The following link is for a nuclear fractionation protocol. The first supernatant will be the cytoplasmic fraction: http://www.abcam.com/assets/pdf/protocols/Nuclear%20fractionation%20protocol.pdf I hope this helps. ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"