The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 50 kDa.
Use at an assay dependent dilution.
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
Post-translational modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [6B1] (ab28036)
Ab28036, at a dilution of 1/1000, staining beta II Tubulin (green) in methanol fixed HeLa cells by Immunofluoresence.
Immunocytochemistry/ Immunofluorescence - beta II Tubulin antibody [6B1] (ab28036)
Ab28036, at a dilution of 1/1000, staining beta II Tubulin (green) in methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence.
References for Anti-beta II Tubulin antibody [6B1] (ab28036)
This product has been referenced in:
Kuznetsov AV et al. H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochim Biophys Acta1853:276-84 (2015).
Read more (PubMed: 25450968) »
Yancey DM et al. Cardiomyocyte mitochondrial oxidative stress and cytoskeletal breakdown in the heart with a primary volume overload. Am J Physiol Heart Circ Physiol308:H651-63 (2015).
Read more (PubMed: 25599572) »