• Product nameAnti-beta II Tubulin antibody [6B1]
    See all beta II Tubulin primary antibodies
  • Description
    Mouse monoclonal [6B1] to beta II Tubulin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, all Mammals
  • Immunogen

    Synthetic peptide, corresponding to amino acids CEEEEGEDEA at the C terminus of human beta II Tubulin.



Our Abpromise guarantee covers the use of ab28036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF Use at an assay dependent dilution.


  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA506K6.1 antibody
    • Beta2 antibody
    • class II beta tubulin isotype antibody
    • class IIa beta-tubulin antibody
    • class IIb beta-tubulin antibody
    • Class IVb beta tubulin antibody
    • dJ40E16.7 antibody
    • DKFZp566F223 antibody
    • FLJ98847 antibody
    • M(beta)2 antibody
    • MGC8685 antibody
    • OTTHUMP00000015956 antibody
    • OTTHUMP00000015964 antibody
    • TBB4B_HUMAN antibody
    • TUBB 2 antibody
    • TUBB 2A antibody
    • TUBB 2C antibody
    • TUBB antibody
    • TUBB PARALOG antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB2B antibody
    • TUBB2C antibody
    • Tubb4b antibody
    • Tubulin beta 2 antibody
    • Tubulin beta 2 chain antibody
    • Tubulin beta 2A antibody
    • Tubulin beta 2A chain antibody
    • Tubulin beta 2B antibody
    • Tubulin beta 2B chain antibody
    • Tubulin beta 2C antibody
    • Tubulin beta polypeptide antibody
    • Tubulin beta polypeptide 2 antibody
    • Tubulin beta polypeptide paralog antibody
    • Tubulin beta-2 chain antibody
    • Tubulin beta-2C chain antibody
    • Tubulin beta-4B chain antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta 2B class IIb antibody
    • tubulin, beta 4B class IVb antibody
    • Tubulin, beta, class IVB antibody
    • Tubulin, beta-4B antibody
    see all

Anti-beta II Tubulin antibody [6B1] images

  • Ab28036, at a dilution of 1/1000, staining beta II Tubulin (green) in methanol fixed HeLa cells by Immunofluoresence.
  • Ab28036, at a dilution of 1/1000, staining beta II Tubulin (green) in methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence.

References for Anti-beta II Tubulin antibody [6B1] (ab28036)

This product has been referenced in:
  • Kuznetsov AV  et al. H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochim Biophys Acta 1853:276-84 (2015). WB, IF ; Rat . Read more (PubMed: 25450968) »
  • Yancey DM  et al. Cardiomyocyte mitochondrial oxidative stress and cytoskeletal breakdown in the heart with a primary volume overload. Am J Physiol Heart Circ Physiol 308:H651-63 (2015). IHC-P ; Rat . Read more (PubMed: 25599572) »

See all 4 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Rat Tissue lysate - whole (gastroc)
Specification gastroc
Total protein in input 800 µg

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Submitted Feb 24 2015