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Three different synthetic peptides conjugated to KLH. These synthetic peptides corresponded to different regions of the beta III Tubulin gene product, but are shared between human and rat.
Equal volumes of three different affinity-purified anti-peptide antibodies were combined to make this product.
Our Abpromise guarantee covers the use of ab41489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/30000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|ICC/IF||1/1000 - 1/2000.|
|IHC (PFA fixed)||1/1000 - 1/2000.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23826406|
Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GFP, Blue: DAPI.
Immunocytochemistry/Immunofluorescence analysis of SpH21 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GAD67, Blue - DAPI.
Immunofluorescence analysis of HPC and PBMC blood-derived iPSC clones, staining beta III Tubulin (green) with ab41489.
Cells were fixed with 4% PFA and blocked for 30 minutes in PBS with 5% fetal bovine serum. Cells were incubated with primary antibody overnight at 4°C. A FITC-conjugated donkey anti-chicken IgG was used as the secondary antibody.