Anti-beta III Tubulin antibody [TUJ-1] (ab14545)

Overview

  • Product nameAnti-beta III Tubulin antibody [TUJ-1]
    See all beta III Tubulin primary antibodies
  • Description
    Mouse monoclonal [TUJ-1] to beta III Tubulin
  • Tested applicationsIHC-P, ICC/IF, IHC-Fr, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Monkey, Quail, Dogfish/Catshark
  • Immunogen

    Tissue, cells or virus corresponding to Rat beta III Tubulin.

  • Positive control
    • mouse, rat brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab14545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/1000.
IHC-Fr 1/1000.
ICC Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • Tissue specificityExpression is primarily restricted to central and peripheral nervous system.
  • Involvement in diseaseDefects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta 3 tubulin antibody
    • beta 4 antibody
    • beta-4 antibody
    • CDCBM antibody
    • CFEOM3A antibody
    • M(beta)3 antibody
    • M(beta)6 antibody
    • MC1R antibody
    • Neuron Specific Class III beta Tubulin antibody
    • Neuron-specific class III beta-tubulin antibody
    • QccE-11995 antibody
    • QccE-15186 antibody
    • TBB3_HUMAN antibody
    • TUBB 3 antibody
    • TUBB 4 antibody
    • TUBB3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    see all

Anti-beta III Tubulin antibody [TUJ-1] images

  • ab14545 (1/1000) staining neuronal processes in mouse primary cortical cell culture.

  • ab14545 at 1/1000 staining chicken E7 body Transverse section tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary antibody.

    See Abreview

  • ab14575 at 1/4000 staining rat brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incuabtion with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.

    See Abreview

  • ab14545 at 1/5000 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was perfomred prior to incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary antibody. The mouse cerebellum gives an identical staining to rat, although mouse sections have been found to require a higher dilution of antibody.

    See Abreview

  • Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for Neuron specific beta III Tubulin antibody [TUJ-1]- Neuronal Marker (ab14545) on Dogfish/Catshark Tissue sections (Whole head mid-saggital section). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab14545 used at 1/4000 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti Mouse Ig (1/200).
  • Immunohistochemistical detection (formaldehyde/PFA-fixed paraffin-embedded sections) of neuron specific beta III Tubulin antibody [TUJ-1] - Neuronal Marker (ab14545) on Quail Tissue sections (nasopharynx or ear). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab14545 incubated at 1/3000 for 2 hours at RT. Secondary Antibody: Biotin labelled Goat anti Mouse Igs.
  • The image shows a cross section through the e13.5 mouse retina. Green cells are positive for  neuronal beta III tubulin. This picture was kindly supplied as part of the review submitted by Dean Griffiths.
  • ab14545 at a dilution of 1/1000, staining TUJ-1 (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images [A] and [B] show filamentous staining observed using a 20x objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

References for Anti-beta III Tubulin antibody [TUJ-1] (ab14545)

This product has been referenced in:
  • Li Y  et al. Topoisomerase IIbeta is required for proper retinal development and survival of postmitotic cells. Biol Open 3:172-84 (2014). ICC/IF ; Mouse . Read more (PubMed: 24463367) »
  • Hagelkruys A  et al. A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain development in the absence of its paralog. Development 141:604-16 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24449838) »

See all 17 Publications for this product

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While I am not aware of the causes for removal of ab14545 from our catalog, we do highly recommend using ab78078 as a replacement product. Ab78078 is tested in and guaranteed to work in a number of applications...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Monkey Tissue sections (DRG)
Specification DRG
Fixative Bouin's
Permeabilization Yes - triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (differentiated neural stem cells)
Loading amount 25 µg
Specification differentiated neural stem cells
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 23 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (naive mouse brain)
Specification naive mouse brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% Triton X-100
Blocking step TNB (other company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Quail Tissue sections (nasopharynx or ear?)
Specification nasopharynx or ear?
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citric acid pH10
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 03 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dogfish/Catshark Tissue sections (Whole head mid-saggital section)
Specification Whole head mid-saggital section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 27 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Jejunum)
Specification Jejunum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 29 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Cor1 Neuronal stem cells)
Specification Cor1 Neuronal stem cells
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

Submitted May 22 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 06 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"