Overview

  • Product nameAnti-Beta Lactamase antibody [8A5.A10]
    See all Beta Lactamase primary antibodies
  • Description
    Mouse monoclonal [8A5.A10] to Beta Lactamase
  • SpecificityThis antibody specifically recognizes TEM type beta lactamases.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to Beta Lactamase.
    Sequence:

    MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

  • General notesDilute in PBS or medium which is identical to that used in the assay system.

Properties

Applications

Our Abpromise guarantee covers the use of ab12251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.)

Target

  • RelevanceThe beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.
  • Alternative names
    • AmpA antibody
    • AmpC antibody
    • Cephalosporinase antibody

References for Anti-Beta Lactamase antibody [8A5.A10] (ab12251)

This product has been referenced in:
  • Fantappiè L  et al. Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen. J Extracell Vesicles 3:N/A (2014). WB . Read more (PubMed: 25147647) »
  • Heggeset TM  et al. Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli. Appl Environ Microbiol 79:559-68 (2013). WB . Read more (PubMed: 23144128) »

See all 8 Publications for this product

Product Wall

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ab12251 and ab12252 were derived from mice that were immunized with the full-length beta lactamse. The immunogen of ab12251 was recombinant TEM-1 beta-lactamase while ab12252 was native Type IV beta-lactamase from ...

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ab12251's immunogen is 5'-His-tagged E.coli TEM-1 beta lactamase therefore this antibody is specific to TEM-1 beta lactamases. I have compared the sequence similarity between TEM-1 beta lactamase and ab104926 and ...

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As discussed over the phone, ab12251 and ab97678 are both mouse monoclonals against beta-lactamase. Whilst I cannot find any reference of ab97678 being used in detecting beta-galctosidase expressed in Le...

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Thank you for contacting us. The lactamases appear to be well-conserved, and the antibodies ab97678 and ab12251 are likely to react with a variety of them. They may possibly recognize your sequence but the epitopes they recognize have not been mappe...

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To the best of our knowledge, this antibody recognizes all TEM-type beta lactamases. Data is limited to our own work with TEM-1 and reports from customers who have worked with various strains of E. coli. To date, we have not been informed by any of o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"