Overview

  • Product nameAnti-beta Sarcoglycan antibody
    See all beta Sarcoglycan primary antibodies
  • Description
    Rabbit polyclonal to beta Sarcoglycan
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (

    FSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGN

    ) of Human beta Sarcoglycan (NP_000223)

  • Positive control
    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificityHighest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.
  • Involvement in diseaseLimb-girdle muscular dystrophy 2E
  • Sequence similaritiesBelongs to the sarcoglycan beta/delta/gamma/zeta family.
  • Post-translational
    modifications
    Disulfide bonds are present.
  • Cellular localizationCell membrane, sarcolemma. Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 43 kDa dystrophin associated glycoprotein antibody
    • 43 kDa dystrophin-associated glycoprotein antibody
    • 43DAG antibody
    • A3b antibody
    • Beta SG antibody
    • Beta-sarcoglycan antibody
    • Beta-SG antibody
    • dystrophin-associated glycoprotein, 43-kD antibody
    • LGMD2E antibody
    • Limb girdle muscular dystrophy 2E (non linked families) antibody
    • Sarcoglycan beta antibody
    • sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) antibody
    • SGC antibody
    • SGCB antibody
    • SGCB_HUMAN antibody
    see all

Anti-beta Sarcoglycan antibody images

  • Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-beta Sarcoglycan antibody (ab83699)

ab83699 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"