Anti-beta Sarcoglycan antibody (ab83699)

Overview

  • Product name
    Anti-beta Sarcoglycan antibody
    See all beta Sarcoglycan primary antibodies
  • Description
    Rabbit polyclonal to beta Sarcoglycan
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (

    FSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGN

    ) of Human beta Sarcoglycan (NP_000223)

  • Positive control
    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Function
    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificity
    Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.
  • Involvement in disease
    Limb-girdle muscular dystrophy 2E
  • Sequence similarities
    Belongs to the sarcoglycan beta/delta/gamma/zeta family.
  • Post-translational
    modifications
    Disulfide bonds are present.
  • Cellular localization
    Cell membrane, sarcolemma. Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 43 kDa dystrophin associated glycoprotein antibody
    • 43 kDa dystrophin-associated glycoprotein antibody
    • 43DAG antibody
    • A3b antibody
    • Beta SG antibody
    • Beta-sarcoglycan antibody
    • Beta-SG antibody
    • dystrophin-associated glycoprotein, 43-kD antibody
    • LGMD2E antibody
    • Limb girdle muscular dystrophy 2E (non linked families) antibody
    • Sarcoglycan beta antibody
    • sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) antibody
    • SGC antibody
    • SGCB antibody
    • SGCB_HUMAN antibody
    see all

Images

  • Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)



    Gel concentration: 12%

References

ab83699 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Cardiac muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE Novex 10% Bis-Tris Protein Gels)
Loading amount
10 µg
Specification
Cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Federica Montanaro

Verified customer

Submitted Apr 13 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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