beta Sodium Potassium ATPase antibody [M17-P5-F11] (ab2873)

Product Name 

beta Sodium Potassium ATPase antibody [M17-P5-F11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [M17-P5-F11] to beta Sodium Potassium ATPase

Immunogen 

Purified lamb kidney beta sodium/potassium ATPase.

Reacts with 
(species key)

Hu, Dog, Pig, Shp

Specificity 

Detects sodium/potassium ATPase beta 1.
This antibody does not recognize the beta 1 or beta 2 subunit in rat.

Epitope 

This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.

Tested applications 
(see key)

IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Immunohistochemistry: 1/100
Western Blot: 1/250
Immunoprecipitation: Assay dependent.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract.

Immunohistochemical staining of the sodium/potassium ATPase yields a pattern consistent with plasma membrane localization.

Cellular localization 

Membrane; single pass type II membrane.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels

Relevance 

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (approximate residues 62-304) of the beta subunit.
The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit a tissue-specific and developmental pattern of expression. The alpha 1 and beta mRNAs are present in all cell types examined whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

M17-P5-F11

Isotype 

IgG2a

Purity 

Ascites

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for beta Sodium Potassium ATPase antibody [M17-P5-F11] (ab2873)

  This product has been used in: (two most recent references)

Turowski P et al. Basement membrane-dependent modification of phenotype and gene expression in human retinal pigment epithelial ARPE-19 cells. Invest Ophthalmol Vis Sci 45:2786-94 (2004). PubMed: 15277505

Sun Y & Ball WJ Determination of Na(+)-K(+)-ATPase alpha- and beta-isoforms and kinetic properties in mammalian liver. Am J Physiol 262:C1491-9 (1992). PubMed: 1319675
If you publish research using ab2873 please let us know so that we can cite the reference on this datasheet.

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