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Product Name
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beta Sodium Potassium ATPase antibody [M17-P5-F11]
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See all beta Sodium Potassium ATPase antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [M17-P5-F11] to beta Sodium Potassium ATPase
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Immunogen
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Purified lamb kidney beta sodium/potassium ATPase.
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Reacts with
(species key)
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Hu, Dog, Pig, Shp
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Specificity
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Detects sodium/potassium ATPase beta 1. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
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Epitope
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This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
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Tested applications
(see key)
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IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Immunohistochemistry: 1/100 Western Blot: 1/250 Immunoprecipitation: Assay dependent. Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract.
Immunohistochemical staining of the sodium/potassium ATPase yields a pattern consistent with plasma membrane localization.
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Cellular localization
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Membrane; single pass type II membrane.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
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Relevance
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The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (approximate residues 62-304) of the beta subunit. The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit a tissue-specific and developmental pattern of expression. The alpha 1 and beta mRNAs are present in all cell types examined whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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M17-P5-F11
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Isotype
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IgG2a
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Purity
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Ascites
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Sodium Potassium ATPase antibody [M17-P5-F11] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for beta Sodium Potassium ATPase antibody [M17-P5-F11] (ab2873)
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This product has been used in: (two most recent references)
Turowski P et al. Basement membrane-dependent modification of phenotype and gene expression in human retinal pigment epithelial ARPE-19 cells. Invest Ophthalmol Vis Sci 45:2786-94 (2004). PubMed: 15277505
Sun Y & Ball WJ Determination of Na(+)-K(+)-ATPase alpha- and beta-isoforms and kinetic properties in mammalian liver. Am J Physiol 262:C1491-9 (1992). PubMed: 1319675
If you publish research using ab2873 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to beta Sodium Potassium ATPase
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