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Anti-beta Sodium Potassium ATPase antibody [M17-P5-F11]
See all beta Sodium Potassium ATPase products (7) ...
Mouse monoclonal [M17-P5-F11] to beta Sodium Potassium ATPase
Detects sodium/potassium ATPase beta 1. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
IHC-Fr, IP, WB, IHC-P, ICC/IFmore details
Reacts with
Sheep, Dog, Human, Pig
Purified lamb kidney beta sodium/potassium ATPase.
This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
PBS with 0.05% sodium azide
Concentration information loading...
Ascites
Monoclonal
M17-P5-F11
IgG2a
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-beta Sodium Potassium ATPase antibody [M17-P5-F11](ab2873)
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Our Abpromise guarantee covers the use of ab2873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/100
IP: Use at an assay dependent dilution.
WB: 1/250
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Found in most tissues.
Belongs to the X(+)/potassium ATPases subunit beta family.
Membrane.
Target information above from: UniProt accessionP05026
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-beta Sodium Potassium ATPase antibody [M17-P5-F11](ab2873)
](/ps/datasheet/images/2/ab2873/beta-Sodium-Potassium-ATPase-Primary-antibodies-ab2873-2.jpg)
IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
See all 3 publications for this product
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](/ps/datasheet/images/2/ab2873/beta-Sodium-Potassium-ATPase-Primary-antibodies-ab2873-2.jpg)
IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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