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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Overview
Product name
Anti-beta Sodium Potassium ATPase antibody [M17-P5-F11]
See all beta Sodium Potassium ATPase products (7) ...
Description
Mouse monoclonal [M17-P5-F11] to beta Sodium Potassium ATPase
Specificity
Detects sodium/potassium ATPase beta 1. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
Tested applications
IHC-Fr, IP, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Sheep, Dog, Human, Pig
Immunogen
Purified lamb kidney beta sodium/potassium ATPase.
Epitope
This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
M17-P5-F11
Isotype
IgG2a
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-beta Sodium Potassium ATPase antibody [M17-P5-F11](ab2873)
 image](/ps/datasheet/images/2/ab2873/beta-Sodium-Potassium-ATPase-Primary-antibodies-ab2873-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab2873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/100IHC-Fr: 1/100
IP: Use at an assay dependent dilution.
WB
WB: 1/250WB: 1/250
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Tissue specificity
Found in most tissues.
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05026
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
see all
Database links
- Entrez Gene: 403966 Dog
- Entrez Gene: 481 Human
- Entrez Gene: 396898 Pig
- Omim: 182330 Human
- SwissProt: P06583 Dog
- SwissProt: P05026 Human
- SwissProt: P05027 Pig
- SwissProt: P05028 Sheep
see all
Anti-beta Sodium Potassium ATPase antibody [M17-P5-F11] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-beta Sodium Potassium ATPase antibody [M17-P5-F11](ab2873)
IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-beta Sodium Potassium ATPase antibody [M17-P5-F11] (ab2873)
This product has been referenced in:
- Suñé Get al. Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney. PLoS One 5:e13930 (2010). WB, ICC/IF, IP; Human.Read more (PubMed: 21085665) »
- Turowski Pet al. Basement membrane-dependent modification of phenotype and gene expression in human retinal pigment epithelial ARPE-19 cells. Invest Ophthalmol Vis Sci 45:2786-94 (2004).Read more (PubMed: 15277505) »
See all 3 publications for this product
Publishing research using ab2873? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"