Anti-beta subunit Cholera Toxin antibody (ab34992)

I have enclosed the requested blots as per our previous conversation. Please let me know If there is anything else I could provide you. I would really like the issue resolved. I want to clearly state the new lot of antibody is not working as the previous one. Thank you. Regards,

ANSWER:

Thank you for contacting us.

Your credit note ID is CNXXXXX. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

I have enclosed the requested blots as per our previous conversation. Please let me know If there is anything else I could provide you. I would really like the issue resolved. I want to clearly state the new lot of antibody is not working as the previous one. Thank you.     Regards,    

ANSWER:

Thank you for getting back to me and for providing some further details of the experiments. I am very sorry to hear that you are dissatisfied with the quality of this product.

According to the originator of ab34992, they tested the suitability of this product for IP in gel diffusion techniques. At this stage, they do not have any supporting published references yet. As soon as we have more information, we will update the on-line datasheet accordingly.

I could certainly arrange a credit note or a refund for you as you requested. Could you please confirm your Abcam Order Number (or your Purchase Order Number), the date or purchase and the name of your Institute.

I look forward to hearing from you

Hi, I ordered Anti-beta subunit Cholera Toxin antibody(ab34992). lot no GR6241-3. I see signal in western blot analysis. But when I used the antibody for Immunoprecipitation, I don't see any binding with my antigen.I am trying to elute the antigen that binds with antibody. The strange thing is I have previously used the same antibody to immunoprecipitate my antigen. Since I ran out of antibody, I ordered  new lot and it doesn't work. What would you advise me to do ? Thanks. regards    

ANSWER:

Thank you for your enquiry regarding ab34992. As my colleague has informed you previously, this antibody has been tested for IP in gel diffusion techniques. The originator, unfortunately, do not have any supporting references. However, they suggest using the antibody at a concentration of around 10 to 50µg/ml for IP. Also it is advisable to titrate around these suggested concentrations in order to find the optimal dilution. Could you please provide some further details of the experiments and an image representing the results with the different bathes? I look forward to hearing from you soon. Thank you for your understanding and co-operation in this matter.    

Would like pre-immune serum from the rabbit that produced ab34992.

ANSWER:

Thank you for your call yesterday and for your patience while I have been in touch with the lab about ab34992.

Unfortunately the pre-immune serum is not available for this antibody. However, we do have rabbit serum and rabbit IgG isotype controls that should be suitable for your experiments-

http://www.abcam.com/Rabbit-Serum-sterile-ab7487.html

http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html

Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

I have a question about ab34992 beta subunit Cholera Toxin antibody. Can ab34992 detect both monometric and pentameric forms of Cholera Toxin beta subunit?

ANSWER:

Thank you for your enquiry. Our antibody to Beta Subunit Cholera Toxin (ab34992) should recognize the beta subunit in both monomeric and pentameric　forms.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.