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Products:Microbiology >> Toxin >> Bacterial Toxin >> Cholera Toxin
Overview
Product name
Anti-beta subunit Cholera Toxin antibody
See all beta subunit Cholera Toxin products (9) ...
Description
Rabbit polyclonal to beta subunit Cholera Toxin
Specificity
Reacts with the beta subunit of Cholera toxin.
Tested applications
ELISA, IHC-FoFr, Immunodiffusion, WBmore details
Cross reactivity
Reacts with
Other
Immunogen
Purified Choleragenoid.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purification notes
>95% pure
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Toxin >> Bacterial Toxin >> Cholera Toxin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/200 - 1/2000.ELISA: 1/200 - 1/2000.
IHC-FoFr: 1/1000(PubMed: 19406878)
ID: Use at an assay dependent concentration.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
Target
Relevance
The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
Cellular localization
Secreted
Alternative names
- Cholera enterotoxin B chain antibody
- Cholera enterotoxin beta chain antibody
- Cholera enterotoxin gamma chain antibody
see all
References for Anti-beta subunit Cholera Toxin antibody (ab34992)
This product has been referenced in:
- Bernardi KMet al. The E3 ubiquitin ligases Hrd1 and gp78 bind to and promote cholera toxin retro-translocation. Mol Biol Cell 21:140-51 (2010). WB.Read more (PubMed: 19864457) »
- Dreesen IAet al. Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection. J Biotechnol 145:273-80 (2010).Read more (PubMed: 19995584) »
See all 3 publications for this product
Publishing research using ab34992? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"