Anti-beta subunit Cholera Toxin antibody [23043] (ab62429)

Overview

  • Product name
    Anti-beta subunit Cholera Toxin antibody [23043]
    See all beta subunit Cholera Toxin primary antibodies
  • Description
    Mouse monoclonal [23043] to beta subunit Cholera Toxin
  • Specificity
    ab62429 is specific for the beta subunit of Cholera Toxin.
  • Tested applications
    Suitable for: IHC-FoFr, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with Vibrio cholerae.
  • Immunogen

    Full length native protein: beta subunit Cholera Toxin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    23043
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Cellular localization
    Secreted
  • Alternative names
    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin beta chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit B antibody
    • Cholera toxin B protein antibody
    • Choleragenoid antibody
    • CTX B antibody
    • CTXB antibody
    • TOX B antibody
    • TOXB antibody
    • VC1456 antibody
    see all

Anti-beta subunit Cholera Toxin antibody [23043] images

  • ab62429 staining beta subunit Cholera Toxin in Mouse eye with optical nerve tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Methanol and blocked with 2% BSA + 5% Donkey serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS-T) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-beta subunit Cholera Toxin antibody [23043] (ab62429)

This product has been referenced in:
  • Hsu TM  et al. Hippocampus ghrelin signaling mediates appetite through lateral hypothalamic orexin pathways. Elife 4:N/A (2015). IHC . Read more (PubMed: 26745307) »
  • Jeong JH  et al. Cholinergic neurons in the dorsomedial hypothalamus regulate mouse brown adipose tissue metabolism. Mol Metab 4:483-92 (2015). IHC . Read more (PubMed: 26042202) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Permeabilization
Yes - 0.3% Triton-X 100
Fixative
Paraformaldehyde
Username

Mr. Vaibhav Konanur

Verified customer

Submitted Oct 14 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Mouse Tissue sections (Mouse eye with optical nerv)
Specification
Mouse eye with optical nerv
Permeabilization
Yes - Methanol
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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