The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/5000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for Flow Cyt.
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
Expressed predominantly in brain; concentrated in presynaptic nerve terminals.
Belongs to the synuclein family.
Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
Toba S et al. Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport. Sci Rep7:16386 (2017).
Read more (PubMed: 29180624) »
Liu C et al. Co-immunoprecipitation with Tau Isoform-specific Antibodies Reveals Distinct Protein Interactions and Highlights a Putative Role for 2N Tau in Disease. J Biol Chem291:8173-88 (2016).
Read more (PubMed: 26861879) »