Overview

  • Product nameAnti-beta TRCP2 antibody
    See all beta TRCP2 primary antibodies
  • Description
    Rabbit polyclonal to beta TRCP2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (LSCLQSMPSV RCLQISNGTS SVIVSRKRPS EGNYQKEKDL CIKYFDQWSE) of human beta TRCP2

  • Positive control
    • 721 B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. SCF(FBXW11) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(FBXW11) mediates the ubiquitination of phosphorylated NFKBIA, which degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. SCF(FBXW11) mediates the ubiquitination of IFNAR1. Targets phosphorylation-dependent degradation of beta-catenin. Involved in the oxidative stress-induced a ubiquitin-mediated decrease in RCAN1. Mediates the degradation of CDC25A induced by ionizing radiation in cells progressing through S phase and thus may function in the intra-S-phase checkpoint. Has an essential role in the control of the clock-dependent transcription via degradation of PER1 and phosphorylated PER2. Is target of human immunodeficiency virus type 1 (HIV-1) protein VPU to polyubiquitinate and deplete BST2 from cells and antagonize its antiviral action.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 7 WD repeats.
  • DomainThe N-terminal D domain mediates homodimerization.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta Transducin Repeat Containing Protein 2 antibody
    • BTRC2 antibody
    • BTRCP2 antibody
    • F-box and WD repeats protein beta-TrCP2 antibody
    • F-box and WD-40 Domain Protein 1B antibody
    • F-box Protein FBW1B antibody
    • F-box/WD repeat-containing protein 11 antibody
    • F-box/WD repeat-containing protein 1B antibody
    • FBW1B antibody
    • FBW1B_HUMAN antibody
    • FBXW11 antibody
    • FBXW1B antibody
    • Homologous to Slimb protein antibody
    • HOS antibody
    see all

Anti-beta TRCP2 antibody images

  • Anti-beta TRCP2 antibody (ab80429) at 1 µg/ml + 721 B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 62 kDa

References for Anti-beta TRCP2 antibody (ab80429)

ab80429 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse ES cells)
Specification mouse ES cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"