Anti-beta Tubulin antibody [2-28-33] (ab121095)

Overview

  • Product nameAnti-beta Tubulin antibody [2-28-33]
    See all beta Tubulin primary antibodies
  • Description
    Mouse monoclonal [2-28-33] to beta Tubulin
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Beta Tubulin isolated from Sarkosyl-resistant ribbons from Strongylocentrouts purpuratus ( Sea urchin) sperm axonemes.

  • Positive control
    • Breast carcinoma
  • General notes


    Excellent as a protein loading control antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab121095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • BetaTubulin antibody
    • TBB5_HUMAN antibody
    • TUBB antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB5 antibody
    • tubulin beta 2A antibody
    • Tubulin beta chain antibody
    • Tubulin beta-5 chain antibody
    see all

References for Anti-beta Tubulin antibody [2-28-33] (ab121095)

ab121095 has not yet been referenced specifically in any publications.

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