Overview

  • Product nameAnti-beta Tubulin antibody
    See all beta Tubulin primary antibodies
  • Description
    Rabbit polyclonal to beta Tubulin
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Rice
    Predicted to work with: Rat, Chicken, Guinea pig, Dog, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea Urchin, Fungi, Xenopus tropicalis
  • Immunogen

    Synthetic peptide:

    NDLVSEYQQYQDATA

    , corresponding to amino acids 416-430 of Human beta Tubulin.

  • Positive control
    • MAD109 cells, Skin and lung, HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab15568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 19509307

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/200. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • Cellular localizationCytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody images

  • All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).

    See Abreview

  • ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).

References for Anti-beta Tubulin antibody (ab15568)

This product has been referenced in:
  • Grice SJ  et al. Dominant, toxic gain-of-function mutations in gars lead to non-cell autonomous neuropathology. Hum Mol Genet 24:4397-406 (2015). Read more (PubMed: 25972375) »
  • Gallo E  et al. Fluorogen-activating-proteins as universal affinity biosensors for immunodetection. Biotechnol Bioeng 111:475-84 (2014). Read more (PubMed: 24122476) »

See all 18 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate pH6
Sample Sea Urchin Tissue sections (Testes)
Specification Testes
Permeabilization No
Fixative Formaldehyde
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Submitted Oct 06 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (IMR32, Human Neuroblatoma)
Specification IMR32, Human Neuroblatoma
Permeabilization Yes - 0.3% triton X 100
Fixative Paraformaldehyde
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Submitted Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jun 26 2013

Thank you for contacting us and your interest in our products.
We would advise trying the antibody at a dilution of 1 ug/mL for both flow cytometry and ICC staining. This is only an initial recommendation, you may need to increase or decrease this...

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Thank you for your reply.

I have returned the Abreview to you for revision.

The notes section should be at the bottom third of the form.

Please let me know if you have any questions.

Thank you for contacting us. I am very glad to hear that you have taken advantage of our 100% Abreview program.

I have found the review which you have submitted and wish to thank you for this. In order to take advantage of the review code,...

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Thank you for your response. We have predicted cross reactivity with this species, but we have not yet tested in Saccharomyces cerevisiae and did not know what results would occur when using this in yeast. While products used in 'predicted to react'...

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Thank you for that information.

I have just a few more questions regarding this. What species are you testing this product against? Do you run these westerns natively or in a reduced and denatured condition?

If we cannot remedy th...

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Application Western blot
Sample Neurospora crassa Cell lysate - whole cell (non)
Loading amount 35 µg
Specification non
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 21 2011

Application Western blot
Sample Sclerotinia sclerotiorum Cell lysate - whole cell (non)
Loading amount 35 µg
Specification non
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 13 2011

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