• Product nameAnti-beta Tubulin antibody
    See all beta Tubulin primary antibodies
  • Description
    Rabbit polyclonal to beta Tubulin
  • SpecificityTubulin beta Antibody detects endogenous levels of total Tubulin beta protein
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from C-terminal of human beta Tubulin

  • Positive control
    • paraffin-embedded human breast carcinoma tissue (IHC) Mouse brain cell extract (WB)



Our Abpromise guarantee covers the use of ab58446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
    • Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • Sequence similaritiesBelongs to the tubulin family.
    • DomainThe highly acidic C-terminal region may bind cations such as calcium.
    • Post-translational
      Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    • Cellular localizationCytoplasm, cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    Anti-beta Tubulin antibody images

    • 1/50 ab58446 staining beta tubulin in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry, with and without blocking peptide.
    • All lanes : Anti-beta Tubulin antibody (ab58446) at 1/500 dilution

      Lane 1 : mouse brain cell extracts
      Lane 2 : mouse brain cell extracts with blocking peptide

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    References for Anti-beta Tubulin antibody (ab58446)

    ab58446 has not yet been referenced specifically in any publications.

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