Recombinant
RabMAb

Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

Overview

  • Product name
    Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker
    See all beta Tubulin primary antibodies
  • Description
    Rabbit monoclonal [EP1331Y] to beta Tubulin - Microtubule Marker
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic peptide within Human beta Tubulin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P07437

  • Positive control
    • WB: PC12 and HeLa whole cell lysates and mouse brain, rat brain, mouse spinal cord and rat spinal cord tissue lysates. IHC-P: Human gastric carcinoma. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP 1/50.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IHC-Fr 1/100.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificity
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Involvement in disease
    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • Sequence similarities
    Belongs to the tubulin family.
  • Domain
    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker images

  • IHC-Fr image of beta Tubulin staining on zebrafish retina sections using ab52901 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Sodium Citrate and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab52901 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • All lanes : Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Spinal Cord (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Rat) Tissue Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size : 50 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ab52901 at 1/250 dilution staining beta Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.

  • Overlay histogram showing HeLa cells stained with ab52901 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52901, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a diminished signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  • ab52901 at 1/100 dilution staining beta Tubulin in HeLa cells by Immunofluorescence.

  • Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/1000 dilution (in PBS +0.5% Tween20 for 2 hours at 23°C) + 293 human embryonic kidney whole cell lysate at 25 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 45 seconds

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 23°C

    See Abreview

References for Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

This product has been referenced in:
  • Wu R  et al. Transgenerational impairment of hippocampal Akt-mTOR signaling and behavioral deficits in the offspring of mice that experience postpartum depression-like illness. Prog Neuropsychopharmacol Biol Psychiatry 73:11-18 (2016). WB ; Mouse . Read more (PubMed: 27693392) »
  • Rangasamy S  et al. Reduced neuronal size and mTOR pathway activity in the Mecp2 A140V Rett syndrome mouse model. F1000Res 5:2269 (2016). WB ; Mouse . Read more (PubMed: 27781091) »

See all 15 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (induced Neuron)
Specification
induced Neuron
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Permeabilization
No
Specification
Cryopreserved embryonic cortical neurons (QBMcells
Fixative
paraformaldehyde with picric acid
Username

Ms. Babben Tinner

Verified customer

Submitted Feb 26 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Retina, Inner plexiform layer, ganglion cell)
Permeabilization
Yes - Triton X
Specification
Retina, Inner plexiform layer, ganglion cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

Submitted Aug 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm Sodium Citrate PH 6.0
Permeabilization
No
Specification
skin tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 12 2013

Thank you for contacting us. The exact epitope is proprietary information, however I can confirm that it is a synthetic peptide from within the following region corresponding to C terminal amino acids 400-430 of Human beta III Tubulin: ...

Read More

thank you for your enquiry. I understand you concerns and it is regrettable you have previously experienced an error on another datasheet. This is unusual and we endevour to ensure that all the information we have on our datasheets and from our ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph9.0
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde
Username

Herr Dr. Markus Kipp

Verified customer

Submitted Mar 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (cultured cortical neurons)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
cultured cortical neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 human embryonic kidney)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
25 µg
Specification
293 human embryonic kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293 human embryonic kidney)
Permeabilization
Yes - 0,5%(w/v) saponin
Specification
293 human embryonic kidney
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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