Anti-beta Tubulin antibody - Loading Control (HRP) (ab21058)

Overview

  • Product nameAnti-beta Tubulin antibody - Loading Control (HRP)
    See all beta Tubulin primary antibodies
  • Description
    Rabbit polyclonal to beta Tubulin - Loading Control (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Tubulin.

    (Peptide available as ab20775.)

  • Positive control
    • HeLa Whole Cell Lysate, A431 Whole Cell Lysate, NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate, MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate, Brain (Mouse) Tissue Lysate, Spinal Cord (Mouse) Tissue Lysate, Ovary (Mouse) Tissue Lysate - normal tissue, PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate, Brain (Rat) Tissue Lysate - normal tissue IHC-P: FFPE normal human colon tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab21058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • BetaTubulin antibody
    • TBB5_HUMAN antibody
    • TUBB antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB5 antibody
    • tubulin beta 2A antibody
    • Tubulin beta chain antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody - Loading Control (HRP) images

  • IHC image of beta Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab21058, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. 

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Tubulin antibody - Loading Control (HRP) (ab21058) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • All lanes : Anti-beta Tubulin antibody - Loading Control (HRP) (ab21058) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)

References for Anti-beta Tubulin antibody - Loading Control (HRP) (ab21058)

This product has been referenced in:
  • Rosales Gerpe MC  et al. N-linked glycosylation protects gammaretroviruses against deamination by APOBEC3 proteins. J Virol 89:2342-57 (2015). WB . Read more (PubMed: 25505062) »
  • Fros JJ  et al. Chikungunya virus non-structural protein 2-mediated host shut-off disables the unfolded protein response. J Gen Virol 96:580-9 (2015). Mouse . Read more (PubMed: 25395592) »

See all 21 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (lymphocyte)
Loading amount 5000 cells
Specification lymphocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Dec 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"