Anti-beta Tubulin antibody, prediluted (ab15569)

Overview

  • Product nameAnti-beta Tubulin antibody, prediluted
    See all beta Tubulin primary antibodies
  • Description
    Rabbit polyclonal to beta Tubulin, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Gerbil, Sea Urchin, Fungi
  • Immunogen

    Synthetic peptide:

    NDLVSEYQQYQDATA

    , corresponding to amino acids 416-430 of Human beta Tubulin.

  • Positive control
    • MAD109 cells, Skin and lung

Properties

Applications

Our Abpromise guarantee covers the use of ab15569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Working dilution, ready to use for 10 minutes at RT.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
    • Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • Sequence similaritiesBelongs to the tubulin family.
    • DomainThe highly acidic C-terminal region may bind cations such as calcium.
    • Post-translational
      modifications
      Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
    • Cellular localizationCytoplasm, cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    Anti-beta Tubulin antibody, prediluted images

    • ab15569 staining beta Tubulin in human lung by Immunohistochemistry (FFPE-sections).

    References for Anti-beta Tubulin antibody, prediluted (ab15569)

    ab15569 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Valve interstitial cells and valve endothelial cel)
    Gel Running Conditions Reduced Non-Denaturing (Native)
    Loading amount 25 µg
    Specification Valve interstitial cells and valve endothelial cel
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C
    Username

    Dr. hessah alshammari

    Verified customer

    Submitted Feb 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"