Anti-beta Tubulin antibody [TU-12] (ab24385)

Overview

  • Product nameAnti-beta Tubulin antibody [TU-12]
    See all beta Tubulin primary antibodies
  • Description
    Mouse monoclonal [TU-12] to beta Tubulin
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    The epitope recognised by this antibody is highly conserved and therefore the antibody cross reacts with a wide range of species.
  • Immunogen

    Full length native protein (purified) of brain microtubule protein MTP-1 (Pig)

  • EpitopeThe antibody reacts with an epitope located within amino acids 345-430 of C-terminal domain of beta-tubulin subunit.
  • Positive control
    • porcine brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab24385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notesICC: Use at an assay dependent dilution. Ab46814 is suitable for ICC/IF as it is the same clone directly fluorescent labelled.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 38 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
    • Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • Sequence similaritiesBelongs to the tubulin family.
    • DomainThe highly acidic C-terminal region may bind cations such as calcium.
    • Post-translational
      modifications
      Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
    • Cellular localizationCytoplasm, cytoskeleton.
    • Information by UniProt
    • Alternative names
      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    References for Anti-beta Tubulin antibody [TU-12] (ab24385)

    ab24385 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab24385.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"