Anti-beta V Tubulin antibody - Carboxyterminal end (ab82366)

Overview

  • Product nameAnti-beta V Tubulin antibody - Carboxyterminal end
    See all beta V Tubulin primary antibodies
  • Description
    Rabbit polyclonal to beta V Tubulin - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human beta V Tubulin (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P07437

  • Positive control
    • A2058 cytoplasmic lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/1000.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • M40 antibody
    • TBB5_HUMAN antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 5 chain antibody
    • Tubulin beta antibody
    • Tubulin beta chain antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta V Tubulin antibody - Carboxyterminal end images

  • Anti-beta V Tubulin antibody - Carboxyterminal end (ab82366) at 1/50 dilution + A2058 cell line lysates at 35 µg

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-beta V Tubulin antibody - Carboxyterminal end (ab82366)

ab82366 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"