Anti-Betacellulin antibody - Aminoterminal end (ab170516)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Betacellulin antibody - Aminoterminal end
See all Betacellulin primary antibodies -
DescriptionRabbit polyclonal to Betacellulin - Aminoterminal end
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Betacellulin aa 24-54 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P35070 -
Positive control
- MDA-MB231 cell lysate, Human pancreas tissue, MDA-MB231 and HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityAmmonium Sulphate Precipitation
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Purification notesab170516 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab170516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/100 - 1/500. Predicted molecular weight: 20 kDa. | |
| ICC/IF | 1/10 - 1/50. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionPotent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.
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Tissue specificitySynthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine.
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Sequence similaritiesContains 1 EGF-like domain.
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Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
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Database links
- Entrez Gene: 685 Human
- Omim: 600345 Human
- SwissProt: P35070 Human
- Unigene: 710156 Human
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Alternative names
- Betacellulin antibody
- Betacellulin precursor antibody
- BTC antibody
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Images
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Western blot - Anti-Betacellulin antibody - Aminoterminal end (ab170516)Anti-Betacellulin antibody - Aminoterminal end (ab170516) at 1/100 dilution + MDA-MB231 cell lysate at 35 µg
Predicted band size: 20 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Betacellulin antibody - Aminoterminal end (ab170516)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-Betacellulin antibody - Aminoterminal end (ab170516)Immunofluorescence analysis of MDA-MB231 cells, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
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Flow Cytometry - Anti-Betacellulin antibody - Aminoterminal end (ab170516)Flow cytometry analysis of HeLa cells labeling Betacellulin (green, right histogram), using ab170516 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.
Protocols
References
ab170516 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"