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Synthetic peptide within Human Betacellulin aa 24-54 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P35070
Our Abpromise guarantee covers the use of ab170516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 20 kDa.|
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescence analysis of MDA-MB231 cells, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Flow cytometry analysis of HeLa cells labeling Betacellulin (green, right histogram), using ab170516 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.
ab170516 has not yet been referenced specifically in any publications.