Anti-Betacellulin antibody - Aminoterminal end (ab170516)

Overview

  • Product name
    Anti-Betacellulin antibody - Aminoterminal end
    See all Betacellulin primary antibodies
  • Description
    Rabbit polyclonal to Betacellulin - Aminoterminal end
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Betacellulin aa 24-54 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P35070

  • Positive control
    • MDA-MB231 cell lysate, Human pancreas tissue, MDA-MB231 and HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab170516 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 20 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.
  • Tissue specificity
    Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine.
  • Sequence similarities
    Contains 1 EGF-like domain.
  • Cellular localization
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Betacellulin antibody
    • Betacellulin precursor antibody
    • BTC antibody
    • BTC_HUMAN antibody
    • OTTHUMP00000160600 antibody
    • OTTHUMP00000219057 antibody
    • Probetacellulin antibody
    see all

Images

  • Anti-Betacellulin antibody - Aminoterminal end (ab170516) at 1/100 dilution + MDA-MB231 cell lysate at 35 µg

    Predicted band size: 20 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Immunofluorescence analysis of MDA-MB231 cells, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).

  • Flow cytometry analysis of HeLa cells labeling Betacellulin (green, right histogram), using ab170516 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.

References

ab170516 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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