Anti-BFAR antibody (ab47127)

Overview

• Product nameAnti-BFAR antibodySee all BFAR primary antibodies ...
• Description
  Rabbit polyclonal to BFAR
• Tested applicationsIHC-P, WB, IHC-Fr, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A recombinant protein corresponding to amino acids 1-139 of human BAR was used as immunogen.

• Positive controlCNS, neuronal cell lines.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

Applications

Target

• FunctionApoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.
• Tissue specificityExpressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen.
• Sequence similaritiesContains 1 RING-type zinc finger.
  Contains 1 SAM (sterile alpha motif) domain.
• Cellular localizationEndoplasmic reticulum membrane.

Target information above from: UniProt accession Q9NZS9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51283 Human
  • Entrez Gene: 51283 Human
  • Entrez Gene: 51283 Human
  • SwissProt: Q9NZS9 Human
  • SwissProt: Q9NZS9 Human
  • SwissProt: Q9NZS9 Human
  • Unigene: 435556 Human

• Alternative names
  BAR antibodyBfar antibodyBFAR_HUMAN antibody

  Bifunctional apoptosis inhibitor antibodyBifunctional apoptosis regulator antibodyRING finger protein 47 antibodyRNF47 antibody

  see all

Anti-BFAR antibody images

• 

  Western blot - BFAR antibody (ab47127)
  All lanes : Anti-BFAR antibody (ab47127) at 1/1000 dilution
  
  Lane 1 : BAR Recombinant protein (RP), 25 ug of total protein per lane.
  Lane 2 : Jurkat, 25 ug of total protein per lane.
  Lane 3 : Spleen, 25 ug of total protein per lane.
  Lane 4 : Lymph Node, 25 ug of total protein per lane.
  Lane 5 : Brain, 25 ug of total protein per lane.
  Lane 6 : Prostate, 25 ug of total protein per lane.
  Lane 7 : Testis, 25 ug of total protein per lane.
  Lane 8 : Kidney, 25 ug of total protein per lane.
  
  
  Predicted band size : 53 kDa
  Observed band size : 53 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BFAR antibody (ab47127)
  Formalin-fixed, paraffin-embedded sections of tumor and normal human tissues stained for BAR expression using Ab47127 at 1/2000. A. Pancreatic cancer. B. Normal pancreas. C. Esophageal cancer. D. Normal esophagus. Increased expression of BAR was detected in the tumor, compared to normal tissues. Hematoxylin-eosin counterstain."
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BFAR antibody (ab47127)
  Formalin fixed, paraffin embedded tissue sections of normal human brain stained for BAR expression using ab47127 at 1/2000. A, A1: Cerebellum. B, B1: Medulla. A1 and B1 are higher magnifications of A and B, respectively."