Overview

  • Product nameAnti-BFAR antibody
    See all BFAR primary antibodies
  • Description
    Rabbit polyclonal to BFAR
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A recombinant protein corresponding to amino acids 1-139 of human BAR was used as immunogen.

  • Positive control
    • CNS, neuronal cell lines.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000.
WB 1/1000 - 1/2000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-Fr Use at an assay dependent concentration.
IP 1/50 - 1/200.

Target

  • FunctionApoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.
  • Tissue specificityExpressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen.
  • Sequence similaritiesContains 1 RING-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAR antibody
    • Bfar antibody
    • BFAR_HUMAN antibody
    • Bifunctional apoptosis inhibitor antibody
    • Bifunctional apoptosis regulator antibody
    • RING finger protein 47 antibody
    • RNF47 antibody
    see all

Anti-BFAR antibody images

  • All lanes : Anti-BFAR antibody (ab47127) at 1/1000 dilution

    Lane 1 : BAR Recombinant protein (RP), 25 ug of total protein per lane.
    Lane 2 : Jurkat, 25 ug of total protein per lane.
    Lane 3 : Spleen, 25 ug of total protein per lane.
    Lane 4 : Lymph Node, 25 ug of total protein per lane.
    Lane 5 : Brain, 25 ug of total protein per lane.
    Lane 6 : Prostate, 25 ug of total protein per lane.
    Lane 7 : Testis, 25 ug of total protein per lane.
    Lane 8 : Kidney, 25 ug of total protein per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Formalin-fixed, paraffin-embedded sections of tumor and normal human tissues stained for BAR expression using Ab47127 at 1/2000. A. Pancreatic cancer. B. Normal pancreas. C. Esophageal cancer. D. Normal esophagus. Increased expression of BAR was detected in the tumor, compared to normal tissues. Hematoxylin-eosin counterstain."
  • Formalin fixed, paraffin embedded tissue sections of normal human brain stained for BAR expression using ab47127 at 1/2000. A, A1: Cerebellum. B, B1: Medulla. A1 and B1 are higher magnifications of A and B, respectively."

References for Anti-BFAR antibody (ab47127)

ab47127 has not yet been referenced specifically in any publications.

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Expiration date: February 15th, 2012
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"