Overview

  • Product name
    Anti-BHC80 / PHF21A antibody
    See all BHC80 / PHF21A primary antibodies
  • Description
    Rabbit polyclonal to BHC80 / PHF21A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human BHC80/ PHF21A.

    (Peptide available as ab41630)

  • Positive control
    • ab41631 gave a positive result in the following Tissue Lysates Brain (Human) Brain (Mouse) Brain (Rat)

Properties

Applications

Our Abpromise guarantee covers the use of ab41631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa).

Target

  • Function
    Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.
  • Tissue specificity
    Highly expressed in brain. Expressed at much lower level in other tissues.
  • Sequence similarities
    Contains 1 A.T hook DNA-binding domain.
    Contains 1 PHD-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BHC80a antibody
    • BM-006 antibody
    • BRAF35-HDAC complex protein BHC80 antibody
    • BRAF35/HDAC2 complex (80 kDa) antibody
    • KIAA1696 antibody
    • PF21A_HUMAN antibody
    • PHD finger protein 21A antibody
    • PHF21A antibody
    see all

Images

  • All lanes : Anti-BHC80 / PHF21A antibody (ab41631) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Brain (Rat) Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Lin S  et al. Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. J Biol Chem 288:6238-47 (2013). Read more (PubMed: 23349461) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (brain sections)
Specification
brain sections
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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