Overview

  • Product nameAnti-BHLHB5 antibody
    See all BHLHB5 primary antibodies
  • Description
    Rabbit polyclonal to BHLHB5
  • SpecificityThis antibody is specific for BHLHB5.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Zebrafish
  • Immunogen

    A region within synthetic peptide: LPAGAALCLK YGESASRGSV AESSGGEQSP DDDSDGRCEL VLRAGVADPR, corresponding to amino acids 119-168 of Human BHLHB5

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

  • FunctionInhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA.
  • Tissue specificityBrain-specific, with the highest expression in the cerebellum.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic helix loop helix domain containing class B 5 1 2 5 antibody
    • Basic helix loop helix protein 5 antibody
    • Beta3 antibody
    • BHE22_HUMAN antibody
    • bHLHB5 antibody
    • bHLHe22 antibody
    • CAGL85 antibody
    • Class B basic helix-loop-helix protein 5 antibody
    • Class E basic helix-loop-helix protein 22 antibody
    • Class II bHLH protein BHLHB5 antibody
    • TNRC20 antibody
    • Trinucleotide repeat containing 20 antibody
    • Trinucleotide repeat-containing gene 20 protein antibody
    see all

Anti-BHLHB5 antibody images

  • Lane 1 : MARKER
    Lane 2 : Anti-BHLHB5 antibody (ab49315) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg/ml

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-BHLHB5 antibody (ab49315)

ab49315 has not yet been referenced specifically in any publications.

Product Wall

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