Overview

  • Product name
  • Description
    Rabbit polyclonal to BHLHB5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BHLHB5.

    (Peptide available as ab109071.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse hippocampus; Mouse cerebellum; Mouse cortex; Mouse forebrain; Rat cerebellum.

Properties

Applications

Our Abpromise guarantee covers the use of ab95136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA.
  • Tissue specificity
    Brain-specific, with the highest expression in the cerebellum.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic helix loop helix domain containing class B 5 1 2 5 antibody
    • Basic helix loop helix protein 5 antibody
    • Beta3 antibody
    • Beta3a antibody
    • BHE22_HUMAN antibody
    • bHLHB5 antibody
    • bHLHe22 antibody
    • CAGL85 antibody
    • Class B basic helix-loop-helix protein 5 antibody
    • Class E basic helix-loop-helix protein 22 antibody
    • Class II bHLH protein BHLHB5 antibody
    • TNRC20 antibody
    • Trinucleotide repeat containing 20 antibody
    • Trinucleotide repeat-containing gene 20 protein antibody
    see all

Images

  • All lanes : Anti-BHLHB5 antibody (ab95136) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Hippocampus Tissue Lysate
    Lane 3 : Cerebellum Mouse Tissue Lysate
    Lane 4 : mouse cortex Mouse Tissue Lysate
    Lane 5 : Forebrain (Mouse) Tissue Lysate
    Lane 6 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds

References

ab95136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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