Overview

  • Product nameAnti-BHMT antibody
    See all BHMT primary antibodies
  • Description
    Goat polyclonal to BHMT
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    EQQLKELFEKQK

    with a Cysteine residue linker, corresponding to C terminal amino acids 391-402 of Human BHMT (NP_001704.2).

  • Positive control
    • Human Kidney and Liver tissues; Rat Liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 45 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-BHMT antibody images

  • ab115464 at 2.5 µg/ml staining BHMT in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115464 at 2.5 µg/ml staining BHMT in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-BHMT antibody (ab115464) at 0.03 µg/ml + Rat Liver lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa

References for Anti-BHMT antibody (ab115464)

ab115464 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"