Overview

  • Product name
  • Description
    Goat polyclonal to BHMT
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-EQQLKELFEKQK

    , corresponding to C terminal amino acids 391-402 of Human BHMT (NP_001704.1).

  • Positive control
    • Liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 - 4 µg/ml.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
    • Tissue specificity
      Found exclusively in liver and kidney.
    • Pathway
      Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
      Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
    • Sequence similarities
      Contains 1 Hcy-binding domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Betaine homocysteine methyltransferase antibody
      • Betaine homocysteine S methyltransferase 1 antibody
      • Betaine homocysteine S methyltransferase antibody
      • Betaine--homocysteine S-methyltransferase 1 antibody
      • BHMT antibody
      • BHMT1 antibody
      • BHMT1_HUMAN antibody
      • EC 2.1.1.5 antibody
      • Epididymis secretory sperm binding protein Li 61p antibody
      • HEL S 61p antibody
      see all

    Images

    • Anti-BHMT antibody (ab77555) at 0.03 µg/ml + Rat liver lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

    References

    ab77555 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77555.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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